Question: Finding The Best Hit From Blat Output For A Microsatellite
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gravatar for figo
5.7 years ago by
figo200
figo200 wrote:

Hi All

I have a repeat element sequence (Microsatellite) and I want look this element in the entire genome of a mammal which has been assembled as scaffolds. I ran BLAT on this and got around 9000 hits. Now I want to extract the best hits from the .psl file. As some of the hits are on same scaffolds, how I can filter the best hit from this? .Is there a script or program to do this.

Regards

blat • 1.6k views
ADD COMMENTlink modified 5.7 years ago by Shaojiang Cai100 • written 5.7 years ago by figo200
0
gravatar for Shaojiang Cai
5.7 years ago by
Shaojiang Cai100
Singapore
Shaojiang Cai100 wrote:

I would recommend Microsoft Excel, seriously.

Because best hits is your criteria, there may not be such a script tailored for you.

By sorting the # of matches or # of matches / total length, you can get the hits with longest alignment lengths. Or sorting by the starting position of the reference sequence, you can view hits on some contig based on positions. Please check the PSL format.

ADD COMMENTlink written 5.7 years ago by Shaojiang Cai100
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