Hy. I have tested neutrality on 8 haplotypes with Arlequin software. I have used three different neutrality test at same time Tajima D, Fu's Fs and Ewens-Watterson tests. Tajima D and Fu's they give me p-values = 0.98000 and EW a p-value = 0.0000. How does it possible. Futhermore I have found an article that define significats these values and possible sings of overdominace selection at HLA-A locus :
N: sample size (individuals) k: number of sequences detected Fobs: observed homozygosity Fexp: expected homozygosity p EW: Ewens-Watterson’s p-value theta S: θ estimator based on polymorphic sites (S) theta Π: θ estimator based on the number of pairwise differences Tajima's D: Tajima’s statistic p Tajima: Tajima’s p-value HLA-A Population N k F obs F exp p EW theta S theta Π Tajima's D p Tajima Zulu 186 29 0.064 0.121 0.0034 10.778 20.843 2.709 0.99475
May someone can help me Thank you.