Question: Question Of Calculating Counts For Strand-Specific
0
gravatar for camelbbs
6.3 years ago by
camelbbs650
China
camelbbs650 wrote:

Hi all,

Can anybody address these 3 questions in calculating counts for strand-specific by HTseq-count.

  1. How the HTseq-count calculate counts for strand-specific RNAseq. I want to ask the principle of it.

  2. After I checked the Refseq database, I found about 280 genes have two copies in different strand (and different location). Although most of the genes don't have this problem. While HTseq-count calculate the counts, how does it deal with this issue.

  3. Can I compare the gene expression (rpkm or counts) from the strand-specific library and from unstranded library?

Thanks a lot!!

Cam

rnaseq htseq • 1.8k views
ADD COMMENTlink modified 6.3 years ago • written 6.3 years ago by camelbbs650

Hi Camelbbs. I'm adding this comment to all your questions: Please take some time, before you ask a question, to think more about your problems and most likely sources of answers (manuals, FAQs, Google!, etc.). When you ask a question, include some context, tell us why you ask that question, what result you need, etc. Most of your questions are vague, impossible to answer or you changed them following an answer because it became evident that it was not clear. Cheers.

ADD REPLYlink written 6.2 years ago by Eric Normandeau10k
1
gravatar for Mikael Huss
6.3 years ago by
Mikael Huss4.6k
Stockholm
Mikael Huss4.6k wrote:

Yes, it does. See: http://www-huber.embl.de/users/anders/HTSeq/doc/count.html

By default, it assumes strand-specific RNA-seq. To turn this off (as I usually do since I mostly analyze unstranded), add '-s no' to the command line.

ADD COMMENTlink modified 6.3 years ago • written 6.3 years ago by Mikael Huss4.6k

Thanks but I think that's not I am asking. I modified my question.

ADD REPLYlink written 6.3 years ago by camelbbs650
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