Question: How To To Relate The Otu Abundance Table With Bacterial Composition Data In Mothur (Rda Analysis)
1
gravatar for ARich
6.0 years ago by
ARich60
United States
ARich60 wrote:

Hello Biostar

I have few queries regarding the RDA analysis to discuss. Although I see clear difference in the bacterial community between the samples, I am not able to relate the OTU abundance table with bacterial composition data and my factors (Mutations/Day effect in my case) on the RDA axis.

It would be great if it can be explained on mothur shared file.

Cheers,

• 2.8k views
ADD COMMENTlink modified 6.0 years ago • written 6.0 years ago by ARich60

Can you please be more clear in your question: i.e. I do not understand "I am not able to relate the OTU abundance table with bacterial composition data". Are you wondering how to determine names for your OTUs or do you mean something else?

I use MOTHUR quite frequently and I don't know exactly what you mean by RDA analysis -- is this your constrained redundancy analysis?

Also, have you tried the MOTHUR help forum?

ADD REPLYlink modified 6.0 years ago • written 6.0 years ago by Josh Herr5.6k
0
gravatar for ARich
6.0 years ago by
ARich60
United States
ARich60 wrote:

Hello Josh

I have used Mothur Forum. It doesn't provide the information related to constrained ordination method. It has PCA/Pcoa/NMDS which I can't use because its unconstrained method.

I want to know which otus/Factors/Taxonomic group are responsible for the differences between the groups.

Hope this time its clear :) Thanks

ADD COMMENTlink modified 6.0 years ago • written 6.0 years ago by ARich60

hi ARich, thanks for your clarification. This is not an answer to the question though, and you used a space for an answer. Can you please delete this "answer" and add your clarification as an edit to the question you originally asked? Thanks.

ADD REPLYlink written 6.0 years ago by Josh Herr5.6k
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