Question: What Is The Difference Between Rna-Seq And Transcriptome Sequencing?
3
gravatar for toshnam
8.4 years ago by
toshnam620
Seoul, Republic of Korea
toshnam620 wrote:

Hi all,

I guess they are different.
Are they different?
If so, what is the difference?

Thanks in advance.

rna transcriptome • 4.7k views
ADD COMMENTlink written 8.4 years ago by toshnam620
12
gravatar for David Quigley
8.4 years ago by
David Quigley11k
San Francisco
David Quigley11k wrote:

I think these are synonyms. RNA-seq is a specific means to measure the state of the transcriptome.

ADD COMMENTlink written 8.4 years ago by David Quigley11k

+1 Nice link, and it is always tricky to stick your head out by questions where there might be some semantic nuance that you were not aware of.

ADD REPLYlink written 8.4 years ago by Chris Evelo10.0k
3
gravatar for Chris Evelo
8.4 years ago by
Chris Evelo10.0k
Maastricht, The Netherlands
Chris Evelo10.0k wrote:

There is a very nice [?]description in Wikipedia[?]. There are a few different technologies and approaches but, like David said, the two terms are indeed synonyms as long as you mean whole transcriptome sequencing.

ADD COMMENTlink written 8.4 years ago by Chris Evelo10.0k
3
gravatar for Alex
8.3 years ago by
Alex1.4k
Theodosius Dobzhansky Center for Genome Bioinformatics
Alex1.4k wrote:

I think RNA-Seq is more wide term. RNA-Seq includes transcriptome sequencing (polyadenylated RNA), "all" RNA sequencing (include rRNA and non-coding RNA) and variant for various non-coding small RNA sequencing. The main difference in a method of probe preparation.

ADD COMMENTlink written 8.3 years ago by Alex1.4k
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