Hello All,
I have 5 rat samples (gene expression) which were generated on the ABI Rat Genome Survey microarray platform. I have a flat txt file which looks like this:
ID_REF MDSC Syngenic 1 MDSC Syngenic 2 MDSC Tolerant 1 MDSC Tolerant 2 MDSC Tolerant 3 20693443 656 284.98 395.22 224.09 325.84 20693548 4407.01 348.59 555.02 2789.46 4107.16 20693561 446.78 532.756 496.35 395.62 647 20693609 293.83 451.51 368.19 288.89 1146.5 20693611 524.4 675.54 825.74 460.71 584.98 20693655 1316.27 1439.52 675.43 305.96 669.34 20693664 749.78 476.87 1559.31 286.97 534.55 20693701 543.8 292.02 433.24 795.61 789.59
Can anyone suggest how I can normalize these samples, or does the ABI software already normalize it when giving out a table format of the expression. As far as I know, the table example shown above is raw data form. Many thanks, Manisha
