Solid Mate-Pair Alignment With Bwa

Question

[lin.barnum]

I have aligned SOLiD mate-pair reads using bwa-0.5.9. Now, it is my understanding that SOLiD mate-pair reads are oriented in the same direction

R3 ------>                   F3 ------>

I am using a downstream application that expects the reads to be aligned pointing inwards.

R3 ------>                    <------ F3

Would simply reverse-complementing the F3 reads achieve this? If I have an aligned bam file, could I simply reverse complement the read and reverse the quality string or would I need to change any flags as well? Any program to handle this already in the public domain?

Thanks,

Answer 1

[Ashutosh Pandey]

Kind of answered here:

Paired end Mapping : What is BWA SOLiD Paired End Default Direction (bwa sampe) ?