I am dealing with some De novo RNA-seq data (paired-end). First, I assemblied the reads to contigs using Trinity. Then, I mapped the short reads to the contigs with BWA. In order to calculate the abundance of each transcript, I used RSEM software. However, this software requires the two mates of any alignment to be adjacent. Does anyone know how to do this? The problem has been disturbing me for several days. Thanks in advance.
Question: How To Make Two Mates Of Any Alignment To Be Adjacent
6.2 years ago by
yanhe83 • 0
yanhe83 • 0 wrote:
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