*.Asc Files From Capillary Sequencing
0
0
Entering edit mode
10.2 years ago
onter ▴ 170

I've got a number of *.asc files from some capillary seqeuencing, in which the README proclaims the following format. If anyone has experience with this format, I'd really like to understand how the fragment size relates to the number of nucleotides in the allele. Any input or speculations welcome! I have not been able to find information on this file format anywhere...

AFM240zf4 <--------probe name field at line 1 
(AC)n <--------probe description field 
D20S181 <--------D-number from GDB
Z23780 <--------gene symbol (or Z-number from Genbank)
4 <--------number of fragments 
4 <--------number of alleles (up to 36 alleles) 
20 <--------chromosome (1 to 22, 23 is X, 24 is Y, 25 is pseudoautosome) 
q <--------arm of this chromosome (p, q, b for both or c for centromere)
0.000000E+00 <--------cytogenetic location *
0.000000E+00 <--------cytogenetic location **
0.000000E+00 <--------gene location
5.555556e-01 <--------observed heterozygote frequency 
102193 <--------last update mmddyy 
p <--------status p (published) u (unpublished), all data considered published
0 <--------Reliability 
42 0 0 0 <--------CEPH collaborator code number 
Genotyped <--------interpretation
1.600000E-01 1.560000E-01 1.640000E-01 1.660000E-01 <-- size of fragments 
1 2 3 4 <-- correspondence alleles/fragment
3.888889e-01 2.500000e-01 3.611111e-01 0.000000e+00 <-- allelic frequencies
sequencing • 2.0k views
ADD COMMENT
0
Entering edit mode
ADD REPLY

Login before adding your answer.

Traffic: 2707 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6