Hi all, this is my first question.
I am using the Long RNA-seq from ENCODE/Cold Spring Harbor Lab gene expression data and the normalized average RPKM formula provided here:
score = normalized average RPKM [((RPKM1 + RPKM2)/2) *1000]/max(RPKM1 + RPKM2)/2)
does not make any sense to me.
As an example, an average RPKM value of 0.126744 from the first line of this file: A549_cell_longPolyA_CSHL_GeneGencV7.CSHL_LID8963-021WC-b1.LID8964-022WC-b2.gff cannot be derived from RPKM1 = 1.40735 and RPKM2 = 1.61345.
The reason I want to use this formula is I want to combine the values derived from the Calltech experiments, as the CSHL ones are incomplete.
I would appreciate any help with this.