I have a question regarding prioritizing the variants after annotating them with ANNOVAR. I am interested in selecting only the non-sense mutations. So according to the classical definition, a nonsense mutation is a point mutation in a sequence of DNA that results in a premature stop codon, or a nonsense codon in the transcribed mRNA, and in a truncated, incomplete, and usually nonfunctional protein product (wiki). In annovar out, like the exome_summary.csv file when you filter out the exonic column you can see for non-sense mutations you have both stop-gain and stop loss listed. So for non-sense mutation extraction should I consider both or only the stop gain? Can anyone throw me some idea on this, it will be highly appreciated.