Tophat Both Pairs Must Map Concordantly
0
0
Entering edit mode
10.2 years ago
AW ▴ 350

Hi,

I have run tophat with the following parameters, specifying each reads can only map once and that they must map concordantly.

 tophat2 --solexa1.3-quals -p 12 -r 80 --read-mismatches 0 --max-multihits 1 --no-mixed --no-discordant

Therefore why do I have to following output in the align_summary.txt. Were the reads that mapped multiply discarded? ​

Left reads:
               Input:  23815106
              Mapped:   9138883 (38.4% of input)
            of these:     92429 ( 1.0%) have multiple alignments (77607 have >1)
Right reads:
               Input:  23844741
              Mapped:   9168518 (38.5% of input)
            of these:     92790 ( 1.0%) have multiple alignments (77715 have >1)
38.4% overall read alignment rate.

Aligned pairs:   7091314
     of these:     10745 ( 0.2%) have multiple alignments
          and:    250038 ( 3.5%) are discordant alignments
28.7% concordant pair alignment rate.

Thanks

tophat2 • 2.5k views
ADD COMMENT

Login before adding your answer.

Traffic: 1947 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6