Can I perform a pathway enrichment study on z-scores by fgsea/GSVA package?
Say, if I have 4 samples from the same condition, but collected from different locations. And I want to check for the pathways enriched in each of these 4 samples.
Converting read_counts into z-scores, and then performing a pathway enrichment study on these z-scores, will be right?
Or we need log2FC values only for performing an enrichment study?