Chosing a phylogenetic tree construction method
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13 days ago
Audrey ▴ 50

Hi all,

I would like to follow the evolution of a specific gene cluster among a genus of bacteria. Should I select the whole cluster with multiple genes? Or should I select specific genes among this cluster ?

Moreover, what method of construction would you advise me to use?

Sorry for my ignorance on phylogeny, I am not used to construct a lot of phylogenetic trees..

Thank you for your much appreciated help!!

Audrey

tree phylogenetic method • 73 views
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Entering edit mode
13 days ago
Colaptes ▴ 50

When constructing a phylogeny, the main thing to look for is that the sequences you are aligning are homologous. When you say gene cluster, do you mean a multi-copy gene family? If all the genes in the cluster are orthologs/paralogs (with multiple copies derived via gene duplication, etc) then you will be able to align them all together and get a phylogeny. The only issue would be if the genes in the cluster are so deeply diverged that you can no longer accurately align the sequences. If that is the case, you would be better off to divide them into separate orthogroups that you can align.

There are a lot of different phylogenetic methods, but a good one to start with is maximum likelihood, and the program IQtree is a pretty straightforward way to make a maximum likelihood tree.

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