Good morning everyone I am new to RNAseq, and I have some doubts, I hope some of you can guide me.
What is the difference between the units log2 (tpm + 0.001) and log2 (norm_count + 1). I was checking and it seems that both units indicate that the data is normalized, but the difference is not clear to me. Can you help me, please.
The IsoPct is a unit that represents the percentage of an isoform at the level of RNAseq. Some of you know how it is calculated and how it is interpreted.
The sum of the isoforms (expressed as a percentage: IsoPct), of a gene, represents the expression of a gene in 100%?
NOTE: If you can share an article with me, I would greatly appreciate it.