Is 30X coverage good enough ?

Question

[vpsev3]

Hello,

I would like to know if a 30X human genome sequencing with a "high-throughput MGI DNBSEQ-T7" machine presents good quality?

Approximately, how many false calls will there be in my genome?

Thank you.

[GenoMax]

What you are looking to do? If you are looking for calling SNP's then that should be adequate.

[vpsev3]

I intend to compare it with all the sequences in the ClinVar database referenced as pathogenic, and look for indel / duplications in my genome

Answer 1

[4galaxy77]

This is something I have looked at in my own work a bit. For SNP discovery - 30x is fine. For indel discovery, it's probably still fine but you do lose some sensitivity.