MAST with UMI data
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3 months ago
Wocher33 • 0

Hi all,

I want to find differentially expressed genes for two conditions and I saw that MAST is integrated to Seurat in the FindMarker() function. In the MAST vignette log(CPM+1) normalized data are suggested as input.

Is UMI data suitable for MAST?

Is data normalized with the "Seurat method" suitable as input to MAST (division by a size factor, multiplication with 10,000 and log(+1) transformation) ?

A similar question has been asked here 3.5 years but I found the answer not 100% satisfactory

Thanks in advance to everyone thinking about my questions

scRNAseq Seurat MAST • 169 views
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