I want to find differentially expressed genes for two conditions and I saw that MAST is integrated to Seurat in the FindMarker() function. In the MAST vignette log(CPM+1) normalized data are suggested as input.
Is UMI data suitable for MAST?
Is data normalized with the "Seurat method" suitable as input to MAST (division by a size factor, multiplication with 10,000 and log(+1) transformation) ?
A similar question has been asked here 3.5 years but I found the answer not 100% satisfactory
Thanks in advance to everyone thinking about my questions