RNA-seq normalisation

Question

[raavi21198]

Hello members, I have downloaded SRA files and converted them to fastq files. I wish to work on RNA-seq normalisation using these fastq files without converting them to read counts. Please let me know how to do normalisation of these files

Answer 1

[ATpoint]

It sounds like you need to get a basic background first.

RNA Sequencing Data: Hitchhiker’s Guide to Expression Analysis

This countains a basic heads-up on the relevant basics.

[raavi21198]

Thank you

Answer 2

[Zhilong Jia]

GREIN : GEO RNA-seq Experiments Interactive Navigator can give you the normalized read counts after your inputs GSE id.

[raavi21198]

Thank you I shall check this one