I have a serious problem with my data
I want to do the Denovo assembly genome with size genome(AFTER TRIMM):2.6 GIG and coverage of 10 of Buffalo.
My server ram is 132 Gig and I can't use another server with the highest ram. When running the script I receive a ram space error.
Dear Arezou, irrespective of the assembler (which you don't mention), I fear your RAM will limit your success. In addition for a denovo assembly of a mammal genome, 10x coverage might be a bit on the low end, the result might disappointing, though there's more qualified people on this forum.
Instead I'd recommend remapping to an existing assembly, which likely yields more success. Still you can try to reassemble the unmapped reads, just in to sure you didn't miss something crucial. Make sure to quality control those reads, so you don't assemble mostly low quality sequence.