I did a KEGG annotation of RNAseq data. I want do do GO enrichment next.
I do have the EC numbers of the genes of interest, so I converted the EC numbers to GO terms using EC2GO. But I only get one GO per EC term. Can I perform GO enrichment analyses using only a single GO term? Ive seen that some enzymes/proteins have multiple GO terms.
Or what should I do? Any help would greatly appreciated.