scRNAseq STAR create index how to set --sjdbOverhang
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10 weeks ago
Binghong ▴ 20

hello everyone, I want to create index before align read using STAR, the sjdbOverhang is described as ReadLength-1, so does it mean I should set 149 if the data is from Illumina 2×150bp? thanks

scRNAseq STAR sjdbOverhang • 108 views
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