Entering edit mode
2.7 years ago
eschwei
•
0
Hi,
we are looking for somatic hypermutation in a single lymphocyte receptor: we have fastq and bam files mapped using our 400 bp reference sequence, and used ivar to analyse mutation frequencies at DNA level. Is it possible to translate each read using our reference file with its frame information, then analyse variation at protein level? We would also like to know the distribution of the number of mutations per reads. Thank you for your help!
Use keywords as tags - repeating the entire question as tags is useless and even counter-productive. I've fixed it this time, but please be more careful in the future.
Sorry and thanks (a bit new to this)