Why do annotating the same functionality with different databases produce different results?
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5 weeks ago
1184982527 ▴ 10

Hello,

I made functional annotation associated with nitrogen metabolism for metagenomic data using KofamScan(https://www.genome.jp/tools/kofamkoala/) ,NCyc(https://github.com/qichao1984/NCyc)and eggnog-mapper. But these results are significantly different. For example, the ORFs that are annotatated as gene nirK using three methods are different. Why is it so?

Thank you.

kegg annotation eggnog metagenomic • 207 views
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Entering edit mode
5 weeks ago

Different resources may have different notions of what a gene is (they may use different references), they use different sources of information and collect and aggregate the information in different ways (for example by having different requirements/confidence levels). The result is that different databases contain different annotations.

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