My research group had completed an analysis on tRNA levels before I joined to study.
The aim was to compare tRNA abundances of healthy and diseased groups and they have raw data from bulk RNA sequencing. However, bulk RNA-seq results from healthy group are from endothelial cells; whereas bulk RNA-seq results from diseased group are from peripheral blood. They calculated tRNA abundances based on different cell types.
I disagree about the reliability of the comparison of such results, because difference in cell types can affect tRNA abundances, irrelevant from health state.
Do you think such comparison is valid?
I would be grateful if you could share your ideas.
Thanks a lot,