Hi, currently, I am trying to build an accurate transcript model of non-model species.
I have performed CAGE-seq, Iso-seq and strand specific RNA-seq on the same sample.
The problem is, the Iso-seq library kit my service provider used did not employ 5' cap selection.Thus, 5' sequences of my Iso-seq data were incomplete.
Now, I am wondering if CAGE-seq and strand-specific RNA-seq data could complement the Iso-seq data.
However, so far I have not found tools which can integrate CAGE-seq and other seq data.
Is there any tool to combine CAGE-seq data, Iso-seq data and RNAseq data?
Any suggestions would be appreciated.