Entering edit mode
19 months ago
Umer • 0
This might seem like a pretty nieve question. Kindly help me with this. I've downloaded whole cattle genome from NCBI accession number "SRX195354". As per my understanding it is a paired-end data for Holstein goldwyn cattle. Questions i want to ask are
- Is this really paired-end data? both files with accession numbers SRR592656 and SRR592657 represent the Forward and Reverse data ?
- How to convert these SRR files to fastq file? I've used fastq-dump without specifying any parameters on both SRR files to obtain fastq files.
- Running FastQC on both fastq files shows that the reads are of really bad quality ( below 20 scores ). Am I missing any specific way of dumping fastq data from SRR files.
Any help will be greatly helpful for me in this regard. THank You.
This is color-space data. Stay away if you can. Probably not worth the hassle of dealing with this type of data.
How did you find out the data type that its color space ?
That information is in the SRA accession record.