Entering edit mode
2.5 years ago
Azade
▴
20
Hi all, I am trying to study the differential expression of my interest genes in colon cancer. First, I've downloaded the RNA-Seq raw counts from TCGA and have built the count matrix. Now I normalized the raw counts by DESeq2 and want to know what should I do in this step? Do I have to separate the interest genes from my count matrix and then perform differential expression analysis for them? or build a shortlist based on the logFC and adj. p-value and then separate my genes if they exist in that shortlist?? Thanks for any help
You should always let the entire dataset guide the analysis.
RNA-SEQ where only a subset of genes is of interest