News:EpiDiverse Toolkit: a pipeline suite for the analysis of bisulfite sequencing data in ecological plant epigenetics
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EpiDiverse Toolkit: a pipeline suite for the analysis of bisulfite sequencing data in ecological plant epigenetics

Link: https://academic.oup.com/nargab/article/3/4/lqab106/6426027

ABSTRACT

The expanding scope and scale of next generation sequencing experiments in ecological plant epigenetics brings new challenges for computational analysis. Existing tools built for model data may not address the needs of users looking to apply these techniques to non-model species, particularly on a population or community level. Here we present a toolkit suitable for plant ecologists working with whole genome bisulfite sequencing; it includes pipelines for mapping, the calling of methylation values and differential methylation between groups, epigenome-wide association studies, and a novel implementation for both variant calling and discriminating between genetic and epigenetic variation.

Adam Nunn, Sultan Nilay Can, Christian Otto, Mario Fasold, Bárbara Díez Rodríguez, Noé Fernández-Pozo, Stefan A Rensing, Peter F Stadler, David Langenberger, EpiDiverse Toolkit: a pipeline suite for the analysis of bisulfite sequencing data in ecological plant epigenetics, NAR Genomics and Bioinformatics, Volume 3, Issue 4, December 2021, lqab106, https://doi.org/10.1093/nargab/lqab106

NGS toolit epigenetics • 510 views
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