How can I makedatabase for env_nt.tar.gz (from NCBI ftp site) in Diamond

Question

[nonaddldy]

I unzip series of env_nt*.tar.gz, and put them into the integrated files according to the format; When I applied "diamond makedb", the output was like the first figure showed "No such file or directory....".

When I did not unzip the .gz files, the output was like the second figure showed "FASTA format error: Missing '>' at record start".

So should I transfer the .gz file into FASTA format?

Answer 1

[GenoMax]

You do not need to. If you upgrade to the latest version of DIAMOND it is able to use NCBI blast pre-formatted databases (see: Creation of subset of diamond database ) Make sure you upgrade to v.2.0.8 or better.

[nonaddldy]

Thanks a lot.