Tandem Mass Tag (TMT) proteomics data analysis

Question

[F]

Hi everybody,

I am approaching to analyse TMT proteomics data. For the subsequent data analysis, I was wondering about the use of an internal reference channel by pooling all the samples in the run. Is it required for a single MS run? What is the meaning of normalize by an internal pooled sample? If anyone has some interesting link which explains tmt-data analysis I would appreciate the advice!

Thank you, bye

Answer 1

[TerryE.Chenier]

TMT technology can use up to 18 stable isotope tags, enabling the simultaneous comparison of protein levels across 18 different samples. TMT-based quantitative proteomics analysis technique can label virtually all peptide or protein samples, making it a widely adopted method for discovering quantitative protein biomarkers.