The number of controls in RNA-seq

Question

[zizigolu]

Hello

I have RNAseq of miRNAs so the number of tested miRNAs are 1640

I have 2 healthy samples versus 12 cancer samples Does it Statistically make sense to compare two controls with 12 cancers?

Can I trust the p-value in differential expression?

Thank you

[ATpoint]

Cross-posted and answered by both the DESeq2 and edgeR developers: https://support.bioconductor.org/p/9142746/