Tutorial for ROC curve analysis using DE RNA data in R

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2.1 years ago

MS ▴ 30

Hi,

I want to perform ROC analysis using DE RNA data in R studio.

I am new so I want a tutorial that explains all steps from begining how to prepare input data set etc.

thank you

ROC • 588 views

ADD COMMENT • link updated 2.1 years ago by iraun 6.2k • written 2.1 years ago by MS ▴ 30

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What question are you trying to answer with a ROC curve of DE analysis? Do you have some kind of ground truth of the DE status in order to calculate the true positive rate and false positive rate?

ADD REPLY • link 2.1 years ago by iraun 6.2k

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I will investigate the diagnostic potential of mRNAs that show at least 2-fold increase compared to control samples, individually or in groups of two or three, by performing ROC curve analyzes.

I just have DE mRNA list as a result of DeSeq2 and I am planning to add DE status according to FDR, log2FC and p-value.

ADD REPLY • link 2.1 years ago by MS ▴ 30

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Have you considered a MA plot or volcano plot instead?

ADD REPLY • link 2.1 years ago by iraun 6.2k

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