off-target predictions to prioritize list of sgRNA designs
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2.2 years ago
Anand Rao ▴ 630

Greetings!

Is there a tutorial / manual for the command line version of CHOPCHOP that explains

  1. how to design sgRNAs for non-human genome (not available on their web version)
  2. predict off-target(s)
  3. select best performing sgRNAs for each target locus

There are some resources available at

  1. https://bitbucket.org/valenlab/chopchop/src/master/README.md, and

  2. discussion threads under issues at https://bitbucket.org/valenlab/chopchop/issues

Do users here on BioStars know of any additional resources - like an actual tutorial perhaps?

Thanks in advance

sgRNA CRISPR CHOPCHOP • 355 views
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