igv and vcf difference
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2.0 years ago

Hi, I am trying to find point mutations from my bam files but the output is given by vcf and IGV is different. Some mutations in igv file are not in vcf file. So I wonder; when the vcf file is being created is there a filtering based on quality for example?

rnaseq bam mutation vcf igv • 538 views
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Entering edit mode
2.0 years ago
ATpoint 82k

So I wonder; when the vcf file is being created is there a filtering based on quality for example?

Yes, usually variant callers apply a lot of filters. Check the documentation of the caller you used.

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