Question: Cufflinks -G And -G Difference?
1
gravatar for andrew.j.skelton73
5.5 years ago by
London
andrew.j.skelton735.8k wrote:

Hi,

In Cufflinks, when using the -g or -G for the GTF guide, is there a difference in using upper or lower case? (There are two separate entries in the Manual) ... If there is a difference, how could this impact the discovery of novel Isoforms?

Thanks!

cufflinks transcript • 3.8k views
ADD COMMENTlink modified 5.5 years ago by Martombo2.5k • written 5.5 years ago by andrew.j.skelton735.8k
4
gravatar for Martombo
5.5 years ago by
Martombo2.5k
Seville, ES
Martombo2.5k wrote:

with the option -G, cufflinks will not look for any novel transcript. only the reads that can be mapped to the GTF intervals will be retained. with option -g, cufflinkds will look for novel transcripts and it will give an output of both the reads mapped to the GTF file and also the reads mapped somewhere else in the genome, that form a likely transcript

ADD COMMENTlink written 5.5 years ago by Martombo2.5k
1
gravatar for Ashutosh Pandey
5.5 years ago by
Philadelphia
Ashutosh Pandey11k wrote:

-G option won't look for novel isoforms. It will simply ignore alignments that are not present in your annotation file. On the other hand -g option will get you the expression estimates for novel isoforms too.


From Cufflinks manual:

-G/--GTF <reference_annotation.(gtf gff)="">     Tells Cufflinks to use the supplied reference annotation (a GFF file) to estimate isoform expression. It will not assemble novel transcripts, and the program will ignore alignments not structurally compatible with any reference transcript.

-g/--GTF-guide <reference_annotation.(gtf gff)="">     Tells Cufflinks to use the supplied reference annotation (GFF) to guide RABT assembly. Reference transcripts will be tiled with faux-reads to provide additional information in assembly. Output will include all reference transcripts as well as any novel genes and isoforms that are assembled.
ADD COMMENTlink written 5.5 years ago by Ashutosh Pandey11k
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