BLAST ERROR Too many positional arguments (1)

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2.5 years ago

Paula ▴ 60

Hi All!

I created a custom BLAST database and now I am trying to run blastp with a query file.

I ran the following command:

blastp -query sequences_dsrb_sol_1_10.fasta -db /cluster/scratch/pramirez/MAP_DSRB_FILES/second_blastdb_construction/THIS_IS_MY_DATABASE/mdb/dbase -num_threads 16 -max_target_seqs 1 -evalue 1e-30 -out test.txt

And I am getting the following error:

Error:  (CArgException::eSynopsis) Too many positional arguments (1), the offending value: /cluster/scratch/pramirez/MAP_DSRB_FILES/second_blastdb_construction/THIS_IS_MY_DATABASE/mdb/dbase.phr

How can I fix it?

Thanks!

blastp blast • 1.4k views

ADD COMMENT • link updated 18 months ago by Ram 44k • written 2.5 years ago by Paula ▴ 60

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Did you create a protein blast database starting with protein fasta sequence files?

ADD REPLY • link 2.5 years ago by GenoMax 147k

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Hi GenoMax! Yes, I started with protein fasta sequences.

ADD REPLY • link 2.5 years ago by Paula ▴ 60

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