Donwloading latest version of Mouse ref genome
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5 weeks ago
salman_96 ▴ 50

Hi,

I am trying to download the latest version of mus musculus reference genome that is GRCm39. The version I have is GRC38.98. I want to download the fast file but I am confused if I should download the primary assembly (.fa) or the top level (.fa).

Secondly, Is it possible to download it directly to the server. I want to download it using the command line?

Best regards

musculus. Reference Ensembl Mus genome • 259 views
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4 weeks ago
vkkodali_ncbi ★ 3.3k

You can use NCBI Datasets for this. Specifically, you can install the command line tool on your server and download the genome directly there.

For GRCm39, you will want to download everything -- that includes the primary assembly with 19 autosomal, 2 sex chromosomes, and a bunch of unlocalized/unplaced scaffolds, as well as the mitochondrial genome. Mitochondrial sequences are not included in the 'primary assembly' so if you choose just the primary assembly you will miss that. You will want to download top-level sequences -- these are the highest assembled level of sequences. For GRCm39 only top-level sequences are provided by NCBI. Some useful definitions related to genome assemblies can be found here: https://www.ncbi.nlm.nih.gov/assembly/help/

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