Sequence Duplication Levels Failed FastQC

Question

[hpalk42]

I need help interpreting and fixing the Sequence Duplication Levels in my genomic data. We performed Illumina short read sequence for our whole genome. I ran FastQC on the data and it failed on two categories: GC content and Sequence Duplication. How can I adjust and fix the Sequence Duplication Levels as it is causing weird distributions in GenomeScope and KmerGenie. Attached is the Sequence Duplication Level distribution and GC Content distribution:

Answer 1

[GenoMax]

Please see: https://sequencing.qcfail.com/articles/libraries-can-contain-technical-duplication/

Always keep the context of experiment in mind rather than going on "fails" in FastQC.