phylogeny

Question

[saifulislam99121]

Hi,

I have whole genome sequences of 30 E.coli isolates. I want to build phylogeny of these isolates based in their virulence genes.

Is it possible to do this or am i just imagining something absurd?

if it is possible, can someone guide me with the tools name and the pathway?

Thank you.

[Matthias Zepper]

Why shouldn't this be possible?

Extract the sequences from the gene(s) of interest, and then infer the relationships from genetic variation. See e.g. tskit for comprehensive tutorials and tools.

[saifulislam99121]

Thank you for your reply.

I was thinking about all the virulence genes in a scene, not individual. so I need to extract all the virulence genes sequences from all the isolates and build phylogeny? right?

Thank you