Can't create index file of Bam file

Question

[Zahed Alam]

Hello,

I am trying to create a index file of file.bam using Samtools. I have executed this command :

samtools index file.bam  file.bam.bai

But it shows some error while creating this index file. I have attached my screenshot bellow:

Anyone can help me about how can i get rid of this error?

[Arup Ghosh]

samtools arror: [E::hts_idx_push] NO_COOR reads not in a single block at the end 0 -1

[Zahed Alam]

Thank you!