Convert fasta to Fastq/bam

Question

[Zahed Alam]

Hello,

I have a fasta sequence of a organism, now I need to convert the fasta sequence into bam file to add a track in jbrowse genome browser. For that I need Fastq file. can I convert my fasta into fastq?

[samuel.a.odonnell]

You should definitely start by just having a basic look at what these 3 file types contain and it should help clarify what you want. But to be brief, converting fasta to fastq is not performed as this would require quality scores for the sequences in the fasta which are most commonly stored with...fastq files, so the process would be rather circular. Depending on what you want to look at you may not need a fastq file. You can align fasta files to create BAM/SAM files too.

[swbarnes2]

A fasta will have a depth of 1 across the entire length of the sequence. Why do you have to visualize that?