I've been following the guidelines in "RNA-seq by Example" in order to practice analyzing RNA-seq data, but when I try to run the edger.r or deseq2.r code as follows:
I get the following error:
Error in file(file, "rt") : cannot open the connection Calls: read.csv -> read.table -> file In addition: Warning message: In file(file, "rt") : cannot open file 'design.csv': No such file or directory Execution halted
It seems that I'm missing a file called design.csv. Does anyone know what I'm doing wrong or where I can find the design.csv file?