P. falciparum sequencing
1
0
Entering edit mode
8 weeks ago

I am planning an experiment to sequence and analyse Plasmodium falciparum samples for resistance gene detection. Are there any peculiarities to take note of given the high AT richness of the Plasmodium falciparum genome? Also, can anyone recommend sequencing kits for this type of experiment?

AT-rich sequencing falciparum plamsodium • 269 views
ADD COMMENT
3
Entering edit mode
8 weeks ago
ATpoint 66k

My recommendation is to contact a sequencing facility and ask for an end-to-end quote including the library prep and the sequencing. Whole-genome sequencing is standard these days, so all you would need to do is to send in the purified DNA, they do the rest. No need to get started with kits yourself for something as established as that.

ADD COMMENT
0
Entering edit mode

Thanks but we are looking to build capacities in that regard so it is essential that it is on-site. I'm more interested in the analysis and if there are any considerations for working high AT genomes.

ADD REPLY

Login before adding your answer.

Traffic: 2372 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6