Currently, I'm working on a qPCR assay.I normalized the targets according the housekeeping gene Ct mean and Log(2)Ratio for one target is 8 and other one is 5.I have some question Regarding the normalization:
1: is the higher log ratio is good or the lower one for the same gene in two targets?
2: can i calculate the log(10) ratio and what is the difference ?
3.i normalized the Ct mean, is it correct?
1: x= Ct mean( House keeping gene - target)
4: Ratio= log(x2,2)
Any recommendations or solutions would be appreciated.