(ERR): hisat2-align exited with value 134
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Entering edit mode
17 months ago

hi , i am about to using hisat2 in order to align some fastq file and create sam files. i face this err would you pls help me whats the problem ?

here is the command i used:

for i in *_1.fastq; do hisat2 -p 8 -t -x /home/my/bioinformatic/mydocument/refrencegenome/grch38_genome/grch38/genome -1 $i -2 ${i/_1.fastq/_2.fastq} -S ${i/_1.fastq/.sam} ; done

and it was the error :

(ERR): hisat2-align exited with value 134
Time loading forward index: 00:00:03
Time loading reference: 00:00:00
Error while flushing and closing output
terminate called after throwing an instance of 'int'
Error while flushing and closing output
terminate called recursively
Aborted (core dumped)
hisat2 • 331 views
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