Tool:split pooled paired-end fastq using fqkit

Question

[size_t]

fqkit barcode -h
split barcode for PE reads

Usage: fqkit barcode [OPTIONS] --read1 <READ1> --read2 <READ2> --barcode <BAR>

Options:
  -1, --read1 <READ1>     input read1 fastq[.gz] file
  -2, --read2 <READ2>     input read2 fastq[.gz] file, barcode in this file
  -b, --barcode <BAR>     barcode list file, eg: barcode  sample
  -m, --mode <MODE>       barcode position mode, 1:left, 2:right [default: 2]
  -r, --rev_comp          barcode reverse complement
  -e, --error <MISMATCH>  barcode mismatch base count [default: 0]
  -o, --outdir <OUTDIR>   fastq file output dir [default: .]
  -h, --help              Print help information

see here: https://github.com/sharkLoc/fqkit