blast engine error empty blast query vector
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15 months ago

Hi,

I am trying to align a nucleotide sequence against a reference genome using blastn command line version. The command I used was:

blastn -query file.fa -subject genome.fasta -out blast.txt -outfmt "6 sseqid sstart send sseq pident"

I got the error:

blast engine error : Empty CBlastqueryvector

My query file is not empty and there are no duplicate headers. Also there are no special characters or symbols.

Please help me to solve this . Thanks in advance!

Blast alignment • 1.4k views
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Which version of blast are you using?

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blast 2.13.0 installed using anaconda.

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What is the output of head file.fa ?

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same problem here

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