Entering edit mode
19 months ago
Sowmya Pulapet
▴
70
Hi,
I am trying to align a nucleotide sequence against a reference genome using blastn command line version. The command I used was:
blastn -query file.fa -subject genome.fasta -out blast.txt -outfmt "6 sseqid sstart send sseq pident"
I got the error:
blast engine error : Empty CBlastqueryvector
My query file is not empty and there are no duplicate headers. Also there are no special characters or symbols.
Please help me to solve this . Thanks in advance!
Which version of
blast
are you using?blast 2.13.0
installed usinganaconda
.What is the output of
head file.fa
?same problem here