Renormalize after row/column deletion from TPM data in RNA-seq

Question

[Gene_MMP8]

I am working with single-cell data and received the TPM-normalized RNA-seq dataset from my labmate. Once I remove genes or cells during preprocessing, I notice that the data is no longer normalized (colSums from R is not returning 1e06). Is there any workaround to take care of this issue? I want to be able to perform downstream preprocessing with log(TPM+1) data.

Answer 1

[ATpoint]

You don't need to do anything. If data were normalized properly in the first place then subsetting does not change that.