Question: Gene Expression Signatures For Basal Vs Mesenchymal Breast Tumors?
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5.1 years ago by
user790
United States
user790 wrote:

is there a canonical gene expression signature for a 'mesenchymal' breast tumor type versus a 'basal' breast tumor type? unsupervised gene expression clustering in primary breast tumors using PAM50 gene set often reveals five tumor subtypes (basal, her2, luminal a, luminal b and normal-like) but it's not clear how similar or dissimilar basal tumors would be to mesenchymal tumors (i.e. tumors with high vimentin/fibronectin1/snail/twist and low epithelial markers like ecadherin). Is there a canonical gene list for both types and perhaps associated paper that tries to use gene expression to answer what the differences between the two types are?

I could only find (http://www.ncbi.nlm.nih.gov/pubmed/20021961) which does not use genome wide expression analysis and is not conclusive

ADD COMMENTlink modified 5.1 years ago by David Westergaard1.4k • written 5.1 years ago by user790
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gravatar for David Westergaard
5.1 years ago by
Copenhagen, Denmark
David Westergaard1.4k wrote:

I honestly don't think you will find a canonical gene expression signature for anything (do correct me if I'm wrong.) See e.g. this comment from 2003, and that's in controlled cell cultures. Outside the in vitro environment, there are a lot more factors which can influence the gene expression, just to mention a few:

  • Diet
  • Smoking
  • Age
  • Nationality/ethnicity
  • Individual mutations

And the list goes on. That being said, if you have the gene expression data for your tumor lines of interest, you can just go ahead an analyse it yourself. If it's all from Affymetrix chips, you can probably use the frozen RMA approach, otherwise maybe virtualArray is useful.

ADD COMMENTlink written 5.1 years ago by David Westergaard1.4k
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