in an RNA-seq experiment, what threshold would you use to define a set of expressed or active genes in a cell line?
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15 months ago
rls_08 ▴ 40

I am trying to define a set of expressed (active) genes in my cell line for some downstream analysis. What would be your approach for defining this subset of active genes from an RNA-seq experiments? is there a minimum fpkm you would use? some standard deviation from normalized expression?

RNA-seq • 553 views
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This has been asked many times before. Please google for 'fpkm cutoff biostars', 'expressed cutoff biostars' or similar queries. With some effort you find posts spanning the last 10 years.

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